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The preparation before cooking differs depending on if the lobster is alive or dead. Many individuals try to relax the live lobster before cooking it. It is thought the lobster is more relaxed and will not feel as much pain. Organisms were considered AAnPs based on the presence of the puf operon and genes for the synthesis of bacteriochlorophyll a; they were considered rhodopsin supplemented photoheterotrophs based on the presence of gene orthologs for proteorhodopsin or xanthorhodopsin; and they were considered autotrophs based on the presence of RuBisCO and the Calvin pathway. Although these designations were made from draft genome sequences for many strains, the high genome completeness index suggests they are largely correct. Finally, isolates were classified into one of five broad environmental categories based on the source of isolation: Pacific Ocean, Atlantic Ocean, Indian Ocean, polar oceans or eukaryote associated (Table 1).Identification of genes in the Global Ocean SamplingGlobal Ocean Sampling (GOS) sample sites, sampling procedures and sequencing methods are described elsewhere (Rusch et al., 2007; Yooseph et al., 2007).

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