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Once it started it ran a little rough, but it ran. It became so hard to start after that week, that I took the carburator out, completely broke it down and cleaned every piece. I couldn’t get a hold of a Carb kit (I was told nobody works on or has parts for bikes this old), and all of the gaskets looked good so I just put it back together.
Golbeck (anti PsaC). N terminal sequencing of proteins was performed by J. Keen.RNA isolation and RT analysisRNA was isolated from frozen cell pellets as previously described17.
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It was unclear whether GR expression could be induced in airway epithelial cells after the treatment with certain chemical agents or in some abnormal conditions. Therefore, to determine whether dexamethasone or TNF can influence the expression of GR in A549 cell lines, cultures were subjected to treatment with dexamethasone or TNF GR expression was induced by treatment with either dexamethsone or TNF whereas it was undetectable in resting cells (Figure 2). Relatively low doses of dexamethasone (10 9 M) and TNF (1 ng/ml) were able to induce GB expression.