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My point: it is important to observe cultural norms where you travel. Don be a dickhead about it, and be true to yourself without offense. Modern religion might be false and immoral, but that doesn allow us to act like assholes. As a robust Roseobacter phylogeny has yet to emerge from rRNA gene analysis (Buchan et al., 2005; Brinkhoff et al., 2008), we first develop a well supported phylogeny for the lineage from a concatenation of conserved, single copy genes and within this phylogenetic structure we examine the evolutionary relationships as possible constraints on genome content and predictors of the genetic capabilities of each strain. Next, we explore lifestyle strategy (heterotroph, photoheterotroph or autotroph) as a possible driver of genome attributes (that is, imposing or releasing bacteria from constraints on genome content). Finally, we ask whether environmental conditions (defined here by the geographical location of isolation) might best explain the observed differences in genetic traits and the retention or acquisition of specific gene systems.Top of pageMaterials and methodsGenome sequencing, annotation and completeness indexThe 32 Roseobacter genomes publicly available as of 15 August 2008 were used in analyses (see Table 1 for genome details).